Student Projects

Student projects

 

We have intership positions for Msc and Bsc students:

 

Workplan 1: Supervisor Reinout Roscam Abbing PhD student

In the Tytgat Institute, biomedical research on the field of hepatology is performed. We focus on the effects of bile acid signaling on the development of atherosclerosis and inflammation in the liver. Currently, we are investigating the immunosuppressive effects of bile acids in mice and in vitro. We also have projects that focus on the impact of bile acid signaling on cholesterol metabolism. The elective student will perform and assist in mouse experiments in vivo as well as advanced cell culture experiments with immune cells.

He/she will become skilled in:

- Cell culture, FACS analysis and experimental techniques of biochemistry (cholesterol related) and molecular biology.

 

 

 

Workplan 2: Supervisor Joanne Donkers PhD student

In the Tytgat Institute, biomedical research on the field of hepatology is performed. We have performed a screen to find novel NTCP inhibiting compounds that can possibly also block HBV transmission. Currently, the hits are verified in vitro and specificity, dosing etc is determined. Eventually, selected compounds will be tested in vivo using ( knockout) mice.

The elective student will perform and assist in bile acid transport assays, HBV binding studies and possibly eventually in vivo binding studies.

 

He/she will become skilled in:

- Hepatic bile acid kinetics in vitro and in vivo and various means to asses cellular bile acid transport

- (Confocal) microscopy and molecular biology.

 

 

Workplan 3: Supervisor Sandra van de Wiel PhD Student

The research line of our group focuses on the temporal activation of bile acid signaling by intervention of bile acid dynamics in the body. Bile acids have hormone-like functions, with a dynamic concentration that is not steady throughout the day, but follows a rhythm with increased concentrations shortly after a meal. Intervention of this enterohepatic cycle will influence this bile acid dynamics and therefore bile acid signaling. Currently, we are investigating the role of the intestinal bile acid transporter OSTα-OSTβ in disease models of cholestasis. The elective student will perform and assist in mouse experiments in vivo and analyze tissues of wildtype and knockout mice using several techniques.

 

The student will actively participate in a variety of research activities performed in the laboratory which may include handling mice, quantitative PCR, gel electrophoresis, immunohistochemistry, bile salt determination and histological analysis of mouse tissue.

 

 

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